Plasmid

Part:BBa_K5048026:Design

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-14)


plasmid PBAD_AQ_GST


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1210
    Illegal XbaI site found at 323
    Illegal PstI site found at 1190
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1210
    Illegal PstI site found at 1190
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1210
    Illegal BglII site found at 1186
    Illegal BamHI site found at 239
    Illegal BamHI site found at 1176
    Illegal XhoI site found at 1182
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1210
    Illegal XbaI site found at 323
    Illegal PstI site found at 1190
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1210
    Illegal XbaI site found at 323
    Illegal PstI site found at 1190
    Illegal AgeI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1389
    Illegal BsaI site found at 2456
    Illegal SapI site found at 56
    Illegal SapI.rc site found at 3538


Design Notes

We add enterokinase site between Lpp'OmpA and small peptides in order to verify the enzymatic cleavage. We replace the T7 promoter with araBAD promoter to prevent the leakage of promoter.



Source

The insertion fragment is complete synthesis . The backbone is purchased from Genscript.


References